N-Linked Oligosaccharides of Sea Urchin Egg Jelly Induce the Sperm Acrosome Reaction. (fertilization/acrosome reaction/sea urchin/sperm/N-linked oligosaccharides)
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چکیده
منابع مشابه
Evidence for the activation of two different Ca2+ channels during the egg jelly-induced acrosome reaction of sea urchin sperm.
The influx of Ca2+ and its subsequent intracellular increase are required for the acrosome reaction of sea urchin sperm to occur. Spermatozoa must undergo this reaction, which is triggered by the egg jelly, in order to fertilize the egg. Here, the egg jelly-induced Ca2+ influx mechanisms have been studied in sperm loaded with FURA-2 using Mn2+ under the assumption that this divalent ion is an i...
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Considerable evidence is now accumulating from both in vivo and in vitro studies that the oligosaccharide chains of the plant N-linked glycoproteins undergo modification or processing reactions after the oligosaccharide has been transferred from its lipid-linked oligosaccharide intermediate to the protein. These processing reactions occur in the endoplasnic reticulum and Golgi apparatus of the ...
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Incubation of Crithidia fasciculata cells with [U-’“C] glucose led to the synthesis of Man-P-dolichol but not of Glc-P-dolichol. The main and largest dolicho1-P-Plinked oligosaccharide formed was Man7GlcNAcz whether labeling was performed in 5 mM sodium pyruvate or 5.5 mM glucose. The protein-linked, endo-j3-N-acetylglucosaminidase H-sensitive oligosaccharides isolated from mature glycoprote...
متن کاملEgg sialoglycans increase intracellular pH and potentiate the acrosome reaction of sea urchin sperm.
Sea urchin egg jelly (EJ) triggers sperm acrosome reaction (AR), an exocytotic event required for membrane fusion of the gametes. Purified fucose sulfate polymer (FSP) in EJ is one inducer of the AR. Binding of FSP to its receptor regulates opening of two distinct calcium channels and also elevates intracellular pH (pH(i)). EJ also contains sialic acid-rich glycans (sialoglycans (SG)) that were...
متن کاملSialic Acids on N-Linked Oligosaccharides
0-Acetylation of sialic acids has previously been considered an uncommon modification found on certain salivary mucins and neural gangliosides. We show here that glycosidically bound sialic acids from total membranes of rat liver have surprisingly high levels (-20%) of 0-acetylation at the 7or 9-position. This 0-acetylation is further enriched in N-linked oligosaccharides but is barely det...
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ژورنال
عنوان ژورنال: Development, Growth and Differentiation
سال: 1994
ISSN: 0012-1592,1440-169X
DOI: 10.1111/j.1440-169x.1994.00551.x